To apply for this position, please forward a current CV, a cover letter and two support letters via e-mail to Nicolas DEROME email@example.com June 30th, 2017.
Source: PNNL // CC BY-NC-SA 2.0
Qualifications and Requirements
Relevant background in Molecular Evolution, Molecular Biology with related laboratory experience.
Basic molecular techniques such as DNA extraction, PCR, DNA purification and sequencing library preparation.
Background in bioinformatics. Experience in metagenomics will be considered an asset.
Excellent written/verbal communication skills and organisational skills.
Demonstrated ability to work in a team environment.
Gut microbiota, which plays a key role in modulating inflammation, is influenced by many factors, including diet. There is a growing evidence that gut microbiota imbalance contributes to cancer development, either by tumor progression stimulation, chronic activation of inflammation, alteration of tumor microenvironment, induction of genotoxic responses and/or metabolism. Interestingly, LCn3 fatty acids, are anti-inflammatory molecules modulating immune system, and reducing growth of malignant PCa cells in mice.
Objectives & Methods
Therefore, the research objective of this MSc (Master) project is to assess the effect of a diet enriched with LCn3 fatty acids on the human gut microbiota composition (taxonomy and functional repertories) in order characterize the dynamic of bacterial functions associated with inflammation pathways in the course of dietary LCn3 supplementation. Both metabarcoding (16S SSU rRNA gene) and shotgun metagenomic approaches will be considered. Eighty microbiota profiles (Healthy and PCa patients) will be correlated with measures of both systemic and tumor tissue inflammation mediators, in order to examine how the dietary LCn3 supplementation modifies the relationship between gut microbiota composition and inflammation mediators.
This Msc project is part of a Prostate Cancer Canada research project (Movember Discovery Grants 2016 D2016-1336) leaded by Dr Vincent Fradet (Centre Hospitalier Universitaire de Québec, CHUQ).
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